By John N. Abelson, Melvin I. Simon, John R. Sokatch, Robert Adron Harris

This quantity contains chapters at the most up-to-date concepts for measuring metabolites of branched-chain amino acids, many new enzyme assays reminiscent of the dimension of isozymes of acetohydroxacid synthase, and the purification of lately defined enzymes equivalent to isovaleryl-CoA dehydrogenase

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01 M KH2PO4. 45/~m nylon filter and degassed by vacuum pumping bffor~ use. d. X 30 cm length stainless steel column packed with/zBondapak N H 2 (10 #m particle size) (Waters , A sso0ates~ Milford, MA 01757) was used. 0 ml/min, this gave a back pressure of - 300 psi. d. × 25 cm length stainless steel Alltech NH 2 I j. M. Poston, J. BioL Chem. 251, 1859 (1976). 2 See also J. M. Poston, this volume [16] and [17]; D. J. -A. Cotting, this volume [3]. 3D. J. -J. Lin, J. Labelled Compd. Radiopharm. 20, 611 (1983).

The [6] GC-MS ASSAY OF BCH A N D BCK ACIDS 27 quinoxalinols are stable for at least 5 days at room temperature; after 7 days there is a decrease in the ratio of the peak area of the internal standard relative to that of each of the three keto acids. This procedure uses peak areas for quantification. If preferred, it is possible to use peak heights and obtain similar precision and accuracy. 9%), respectively, for the three acids. Reference Ranges One milliliter of human antecubital venous plasma collected after overnight fasting contains 40-80 (mean 63, RSD 25%) nmol of total branched-chain 2-keto acids, 8-16 (mean 13, RSD 22%) nmol of 3methyl-2-ketobutyrate, 20-40 (mean 31, RSD 25%) nmol of 4-methyl-2ketovalerate, and 10-30 (mean 19, RSD 32%) nmol of 3-methyl-2-ketovalerate.

Marfey, CarlsbergRes. Comm. 49, 591 0984). 4 In earlier publications, this compound was called l-fluoro-2,4-dinitrophenyl-L-alaninamide. Since this name does not correspond to any accepted system of nomenclature, the name consistent with IUPAC rules of organic chemical nomenclature is used in this article. We thank Dr. Kurt Loening(ChemicalAbstractsService)for assistance with this nomenclature. 5Pierce ChemicalCompany,"Previews,"p. 9. PierceChemicalCompany,Rockford,Illinois, 1985. 16 ANALYTICAL AND SYNTHETIC METHODS [3] with occasional shaking.

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