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Tay, B. ; Chen, J. ; Han, Z. J. & Khor, K. A. (2007). A novel amperometric biosensor based on ZnO: Co nanoclusters for biosensing glucose. Biosensors and Bioelectronics, 23, 135-139,0956-5663. Zhao, Z. ; Tay, B. ; Chen, J. ; Hu, J. ; Lim, B. C. & Li, G. P. (2007). Large magnetic moment observed in Co-doped ZnO nanocluster-assembled thin films at room temperature. Applied Physics Letters, 90, 152502, 0003-6951. Zhao, Z. ; Tay, B. ; Chen, J. ; Hu, J. ; Sun, X. W. & Tan, S. T. (2005). Optical properties of nanocluster-assembled ZnO thin films by nanocluster-beam deposition.
2009). 5 mg/dl. , 2008) these authors dispersed ZnO nanoparticles in chitosan matrix for film fabrication onto conducting electrode by selective immobilization of cholesterol oxidase to construct the cholesterol biosensor. ZnO-chitosan nanocomposite films deposited on ITO electrode surface were mechanically stable due to excellent film forming ability of chitosan. 3. 41×10−4 Amgdl−1) with a wide linear concentration range from 5-300 mgdl−1 of cholesterol with a detection limit as 5mg dl−1 was achieved.
Nanosized metal oxides based electrochemical enzymatic biosensors have active surfaces that can easily be modified for immobilization of biomolecules. However, this advantage may not apply to many non-oxide semiconductor nanomaterials because their surfaces are not stable in an air environment, which leads to formation of an insulating native oxide layer and may degrade device reliability and sensitivity. Whereas, nanostructured metal oxides based electrochemical transducer surfaces promote the direct electron transfer reactions, amplify and orient the analytic signal of the biorecognition events.