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S. LeQuire, 1. Lipid Res. 10, 433 (1969). ~8Subcellular fractions as isolated are frequently highly structured and contain nonmembranous components within their compartment. For analysis of membranes, such contents can be released from within the compartment, cf. G. Kreibich and D. D. Sabatini, this volume [20]. 59E. L. Benedetti and P. Emelot, in "The Membranes" (A. J. Dalton and F. ), Vol. 4; p. 33. Academic Press, New York, 1968. 30 MULTIPLE FRACTIONS FROM A SINGLE TISSUE [2] Fro. 7. Electron micrographs of Golgi complex fractions from fresh (Fig.

3 rotor. The pellet from band 1 cohsists of smooth microsomes (Golgi-derived elements primarily); the pellet from band 2 is mixed rough and smooth microsomes with some free ribosomes; and the pellet from band 3 consists of rough mierosomes. The high speed soluble fraction resulting from sedimentation of the total microsomhl fraction (the postmicrosomal supernatant) contains the bulk of the nohmembrane-bound proteins of the cytosol (primarily from 6J. Meldolesi, J~ D. Jamieson, and G. E. Palade, J.

Further centrifugations of the 600 g supernatant are carried out in ~L. E. Hokin, Biochim. Biophys. Acta 18, 379 (1955). ~L. J. Greene, C. H. W. Hirs, and G. E. Palade, J. Biol. Chem. 238, 2054 (1963). 3p. Siekevitz and G. E. Palade, 1. Cell Biol. 2, 671 (1956). 4j. D. Jamieson and G. E. Palade, J. Cell Biol. 34, 577 (1967). J. D. Jamieson and G. E. Palade, 1. Cell Biol. 34, 597 (1967). 5 hr Smooth microsomes Rough microsomes FIG. 2. Flow sheet for cell fractionation scheme applied to the guinea pig pancreas.

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