By Dennis E. Vance and Jean E. Vance (Eds.)

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This finding might imply that PE plays different physiological roles in the two bacterial types. 6. Cytokinesis The function of cytokinesis is to divide one cell into two by building a membrane barrier between the two daughter cells. In eukaryotic cells, the interaction of actin filaments with myosin filaments applies tension to the membrane to form a cleavage furrow, which gradually deepens until it encounters the narrow remains of the mitotic spindle between the two nuclei. Phospholipids play an essential role in the division processes in eukaryotic cells.

DnaA is required for initiation of DNA replication and is active in its ATP-bound but not ADP-bound form. In vitro the exchange of ADP for ATP in the complex is greatly stimulated by almost any anionic phospholipid including non-E, coli lipids like PI. An anionic phospholipid-specific positively charged amphipathic helix has been identified in DnaA that appears to direct initial membrane association followed by partial insertion of the protein into the bilayer. The resulting conformational changes alter the ATP/ADP binding properties.

2. Biosynthesis of GPI-anchored proteins: attachment of GPIs to proteins . . . . . . . . . . 3. GPI-anchoring in mammals, parasitic protozoa, and yeast . . . . . . . . . . . . . . . 4. Functions of GPI anchors . . . . . . . . . . . . . . . . . . . . . . . . . . . 9. Future directions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Abbreviations . . . . . . . . . . . . . . . .

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