By Andras Perl
This choice of with no trouble reproducible molecular concepts and similar in vitro/in vivo version platforms can be utilized to discover the motives of autoimmunity, in addition to how most sensible it can be regulated. there are ways to evaluate immunological and biochemical pathways correct for pathogenesis and to set up and examine numerous autoimmune illnesses, together with arthritis, lupus, diabetes, a number of sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and vitiligo.
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Extra resources for Autoimmunity: Methods and Protocols (Methods in Molecular Medicine)
5. Secondary HRP-Labeled Antibodies 1. Goat antirabbit IgG-HRP (Santa Cruz Biotechnology). 2. Antigoat IgG-HRP (Santa Cruz Biotechnology). 3. Antimouse IgG-HRP (Santa Cruz Biotechnology). 3. Kits 1. , Valencia, CA). 2. , Madison, WI). 3. ). 4. , Köln, Germany). 4. RT-PCR, Gene Expression Analysis 1. 2. 3. 4. 5. Ethidium bromide (Sigma Aldrich). Seakem gametic technology grade (GTG) agarose (FMC BioProducts, Rockland, ME). ). PCR beads (Amersham Pharmacia, Piscataway, NJ). X-gal (Sigma-Aldrich).
FACS tubes, 12 × 75 mm polypropylene test tubes without cap (Fisher Scientific). Polylysine-coated Poly Prep slides (Sigma Aldrich). Pap pen with normal large tip (Electron Microscopy Sciences, Washington, PA). 17 mm thick (VWR Scientific, Buffalo Grove, IL). , Hayward, CA). Colorless nail polish from any local store. Saponin (Sigma Aldrich). Digitonin (Sigma Aldrich). Glycine (Bio-Rad). 7. Instruments 1. 2. 3. 4. 5. Dounce homogenizer (Fisher Scientific). Beckman ultracentrifuge and SW41 rotor (Palo Alto, CA).
From: Methods in Molecular Medicine, Vol. 102: Autoimmunity: Methods and Protocols Edited by: A. , Totowa, NJ 49 50 Nambiar et al. 1. 1. TCR c-Chain The T-cell receptor (TCR) c-chain is a vital T-cell signaling molecule that is considered the limiting factor among the eight subunits of the TCR in receptor assembly, transport, and surface expression and is crucial to receptor signaling function (1,2). 7 to more than 8 kb (3). The c-chain exists in multiple forms and membrane fractions with distinct functions in antigen (Ag)-mediated signaling process.