By Andras Perl

This choice of with no trouble reproducible molecular concepts and similar in vitro/in vivo version platforms can be utilized to discover the motives of autoimmunity, in addition to how most sensible it can be regulated. there are ways to evaluate immunological and biochemical pathways correct for pathogenesis and to set up and examine numerous autoimmune illnesses, together with arthritis, lupus, diabetes, a number of sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and vitiligo.

Show description

Read or Download Autoimmunity: Methods and Protocols (Methods in Molecular Medicine) PDF

Similar infectious diseases books

Infectious Disease Management in Animal Shelters

"Infectious disorder administration in Animal Shelters" is a entire advisor to combating, coping with, and treating illness outbreaks in shelters. Emphasizing options for the prevention of disease and mitigation of disorder, this booklet presents certain, useful information about primary ideas of disorder regulate and particular administration of significant ailments affecting canine and cats in staff residing environments.

G-CSF Receptor

The G-CSF receptor is a kind I membrane protein which belongs to the cytokine receptor superfamily, and is in particular expressed in mature neutrophils and neutrophilic precursors. Binding of G-CSF to G-CSFR induces its dimerization. The dimerized receptor transduces development and differentiation signs that are mediated by means of the N-terminal and C-terminal halves of the G-CSFR cytoplasmic zone.

Avian Immunology

The technology underpinning avian immunology is important to realizing simple immunological rules and the outstanding positive aspects of the avian immune approach, as various ideas birds have followed delivers very important evolutionary insights. This booklet presents the main entire photograph of the avian immune approach up to now.

Molecular Immunology

Immune responses consequence from the activation of an intricate community of interacting cells taking place in particular microenvironments. awareness lately has been focussed intensively on a few matters. integrated between those are: the developmental pathways of the 2 significant varieties of lymphocytes, T and B cells; the character in their particular receptors for antigen and in their quite a few accent floor molecules; the following destiny of the cells following stumble upon with antigen awarded as such or on various cells; and the functionality of the effector molecules produced after profitable activation through antigen.

Extra resources for Autoimmunity: Methods and Protocols (Methods in Molecular Medicine)

Example text

5. Secondary HRP-Labeled Antibodies 1. Goat antirabbit IgG-HRP (Santa Cruz Biotechnology). 2. Antigoat IgG-HRP (Santa Cruz Biotechnology). 3. Antimouse IgG-HRP (Santa Cruz Biotechnology). 3. Kits 1. , Valencia, CA). 2. , Madison, WI). 3. ). 4. , Köln, Germany). 4. RT-PCR, Gene Expression Analysis 1. 2. 3. 4. 5. Ethidium bromide (Sigma Aldrich). Seakem gametic technology grade (GTG) agarose (FMC BioProducts, Rockland, ME). ). PCR beads (Amersham Pharmacia, Piscataway, NJ). X-gal (Sigma-Aldrich).

FACS tubes, 12 × 75 mm polypropylene test tubes without cap (Fisher Scientific). Polylysine-coated Poly Prep slides (Sigma Aldrich). Pap pen with normal large tip (Electron Microscopy Sciences, Washington, PA). 17 mm thick (VWR Scientific, Buffalo Grove, IL). , Hayward, CA). Colorless nail polish from any local store. Saponin (Sigma Aldrich). Digitonin (Sigma Aldrich). Glycine (Bio-Rad). 7. Instruments 1. 2. 3. 4. 5. Dounce homogenizer (Fisher Scientific). Beckman ultracentrifuge and SW41 rotor (Palo Alto, CA).

From: Methods in Molecular Medicine, Vol. 102: Autoimmunity: Methods and Protocols Edited by: A. , Totowa, NJ 49 50 Nambiar et al. 1. 1. TCR c-Chain The T-cell receptor (TCR) c-chain is a vital T-cell signaling molecule that is considered the limiting factor among the eight subunits of the TCR in receptor assembly, transport, and surface expression and is crucial to receptor signaling function (1,2). 7 to more than 8 kb (3). The c-chain exists in multiple forms and membrane fractions with distinct functions in antigen (Ag)-mediated signaling process.

Download PDF sample

Rated 4.44 of 5 – based on 42 votes