By Robert Klein, George Enders
This unique try prep consultant allows you to attempt your wisdom of crucial anatomy, histology and cellphone biology techniques for the USMLE Step 1; perform with 500 USMLE Step 1-style questions with referenced solutions; evaluate causes for correct and incorrect answers;and construct self belief, abilities, and information.
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Extra resources for Anatomy, Histology, and Cell Biology PreTest Self-Assessment and Review
The lysate is incubated at 50° for 60 min. 55 vol 2-propanol is added and the sample mixed by inversion until a clot of DNA forms. The clot is collected on a fine glass rod or by low-speed centrifugation. The supernatant is removed as completely as 42 I. ISOLATION, SYNTHESIS, AND DETECTION OF D N A AND R N A possible. 1 ml of CT containing 50 ^g/ml ribonuclease. It is shaken gently at first and then more vigorously as the solution becomes more viscous. When most is dissolved, the sample is incubated for 20 min at 37° to allow the ribonuclease to partially digest the RNA.
All rights of reproduction in any form reserved. 38 I. ISOLATION, SYNTHESIS, AND DETECTION OF D N A AND R N A Extraction of RNA The moment of initial disruption of a cell is the time that ribonucleases are released and can cause degradation of cellular RNA. Cell extraction should therefore be carried out in a solution containing strong denaturing agents and/or other inhibitors of ribonucleases. 0 (the pH of optimal stability for RNA). 5 Although guanidinium thiocyanate is useful as a denaturing agent and ribonuclease inhibitor, 6-10 a mixture of sodium dodecyl sulfate (SDS) and a low concentration of urea 11 is an effective inhibitor of potent leukocyte ribonucleases, which are more difficult to inhibit than pancreatic ribonuclease.
L. Cate, K. L. Ramachandran, M. Wysk, J. C. Voyta, O. J. Murphy, and I. Bronstein, Proc. Natl. Acad. Sei. A. 510 The average exposure time is less than 1 hr and for some applications several minutes are quite sufficient. Exposure time depends greatly on the detection medium: a comparison of X-ray (Fuji Medical) versus Polaroid 57filmrevealed a 50: 1 difference in exposure time. In contrast to Polaroid films, the manufacturers of X-ray films provide no information on sensitiv ity (such as ASA or dynamic range) and therefore more sensitive X-ray films for this particular application may exist.